Hypervolemia suppresses dilutional anaemic injury in a rat model of haemodilution

Abstract Background and Objectives Haemodilution leads to complications in clinical practice. It is exactly unknown whether this damage caused by the fluid or stretching of vascular bed. We aimed compare two different haemodilution techniques at same anaemic level. Methods Normovolemic hypervolemic was performed on twelve adult male Wistar rats. In normovolemic procedure, blood withdrawn instantaneously administered with similar amounts 6% hydroxyethyl starch (HES 130/0.4). Fluid without withdrawing procedure. both models, a 25% haematocrit level targeted kept for 90 min deepen anaemia effect. Besides haemodynamics measurement, renal function (creatinine, urea nitrogen) injury (tissue norepinephrine, malondialdehyde) were evaluated. Also, systemic hypoxia (lactate), oxidative stress (malondialdehyde, ischaemia-modified albumin), inflammation (tumour necrosis factor-alpha [TNF-α]), osmotic stress, adrenal (norepinephrine, epinephrine), (atrial natriuretic peptide [ANP]) assessed. Results Arterial pressure group lower than group. Serum creatinine, nitrogen, lactate levels higher Tissue norepinephrine malondialdehyde ANP, malondialdehyde, albumin, free haemoglobin, syndecan-1, TNF-α groups compared respective baseline. Conclusions may lead hypoxic kidney injury. The state be advantageous if administered. Thus, effect itself can relatively masked.